The ladder is supplied with 6x dna loading dye, and it. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp. Each ladder is provided with a tube of 6x blueorange loading dye. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500. Generuler 1 kb dna ladder thermo fisher scientific. Generuler 1 kb plus dna ladder thermo fisher scientific. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Promega 1kb dna ladder molecular weight marker 1kb dna ladder. Dna ladder is commonly used to determine the size of dna fragments by. The dna includes fragments ranging from 25010,000 base pairs. The format of the msds complies with commission directive 200158ec as amended.
For example, step ladder dna fragments could be in increments of. The ladders are not intended for use in quantitative analysis. Generuler 1kb dna ladder, range 250 to 10,000bpcan be labeled radioactively with t4 polynucleotide kinase seeprotocol. Dna step ladders contain dna bands of defined sizes and equal intensity with fragment lengths at defined increments. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye.
Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. The digested dna includes fragments ranging from 0. G571 application of the substance the mixturelaboratory chemicals details of the supplier of the safety data sheet. The dna ladder consists of proprietary plasmids which. The 500bp fragment is present at increased intensity for easy identification. Straightforward procedure for laboratory production of dna ladder. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Promega 1kb dna ladder molecular weight marker 1kb dna. The 1,000bp and 3,000bp fragments have increased intensity. How can i prepare working solution with 1 kb dna ladder. There have been no changes to product formulation or specifications. Tracking dye does not leave a shadow under uv light.
Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments plus a band at 1,500bp. The 1kb dna ladder is readytouse dna marker bp 1 kb blue is designed for use as molecular weight standard for agarose gel electrophoresis availibility of sample size may be. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Dna step ladders have defined sizes with exact incremental steps between bands. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Promega corporation 2800 woods hollow road madison, wi 53711 u. Large dna size selection with the pronexr sizeselective. How can i prepare working solution with 1 kb dna ladder supplied. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Thermo scientific generuler 1 kb dna ladder 1kb dna ladder. Dna ladders have defined sizes, and are not intended for use in quantitative analysis.
Head to medex supply and check out the promega benchtop 1kb dna ladder. Promega corporation 2800 woods hollow road madison. Agarose gel image of 1kb dna ladder sizeselected using the pronex. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose gel. The 1 kb dna ladder is a readytoload molecular weight marker suitable for dna fragment size determination on gel electrophoresis. Bands within each segment dna ladders have defined sizes, and are not intended for use in quantitative analysis. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. How can i prepare working solution with 1 kb dna ladder supplied by promega.
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